PDA MEA MYA Agar Mushroom Nutrient Agar Powder
All of our products are packed in a controlled environment, each package is enough for 50 plates(20ml per plate), these premixes can also be used for germinating spores. more products soon , please make sure to follow my shop. Mush love!
Potato dextrose agar (PDA) is common microbiological growth media made from potato infusion, and dextrose. Potato dextrose agar is the most widely used medium for growing fungi and bacteria.
Malt Extract Agar is used as a general purpose growth media to isolate and cultivate yeasts and molds from clinical samples, as well as a wide range of environmental sources.
Soy Protein it is often used as a nutrient and vitamin additive for culturing mediums.
Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as a nutrient additive for culture media.
HOW TO USE OUR PREMIXES
Specimen Collection: It is important to take proper cautions when cleaning mold contaminated areas, in order to limit exposure to mold and mold spores. It is recommended that while cleaning an area potentially contaminated with fungi that one wears a N-95 respirator, gloves, and goggles.
1. One package of the Agar premix we make here at RomSpawn is enough for 50 plates (20ml per plate), just add the contents of the package to 1 liter of distilled water and bring to a boil, then use you're preferred method to pour plates.
2. Allow media to warm to room temperature, and the agar surface to dry before inoculating.
3. Using a needle, loop, or swab remove a small amount of mature growth from the area to be cultured. Inoculate and streak the sample as soon as possible after collection.
4. If the specimen to be cultured is on a swab, roll the swab over a small area of the agar surface. Streak for isolation with a sterile loop. If the specimen is collected on a loop or needle, inoculate the plate with fungi in two or three distinct places by pressing the inoculum onto the agar surface. Yeasts should be streaked for isolation using four quadrants.
5. Incubate plates in an inverted position. Once inoculated, media can be incubated aerobically at 25-35ºC. with increased humidity for four weeks or longer. Parafilm may be used to seal the plates to keep moisture from evaporating from the plated media, while still allowing atmospheric circulation
6. Examine plates for typical colonial and hyphal morphology and color.